MYCOBACTERIUM COMPRISING EXPRESSION VECTOR WITH TWO AUXOTROPHIC SELECTION MARKERS AND ITS USE AS VACCINE
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United States of America Patent
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Jul 30, 2015
app pub date -
Jul 2, 2013
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Aug 31, 2012
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Abstract
The invention relates to polynucleotides and recombinant cell strains comprising the polynucleotides and the uses thereof for the delivery of the polypeptides encoded by the polynucleotides to a subject in need thereof. In particular, the invention refers to polynucleotides comprising a polypeptide of interest, auxotrophy-complementing genes and the use thereof in a mycobacterial double auxotrophic host cell to achieve the stable expression of the polypeptide of interest by using an antibiotic-free plasmid selection system.
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