Method of Cross-Linking Hyaluronic Acid with Divinylsulfone
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United States of America Patent
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Issued Date -
Jul 9, 2015
app pub date -
Jan 7, 2015
filing date -
Nov 24, 2004
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Abandoned
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Abstract
The present invention relates to methods of producing a homogenous hydrogel comprising hyaluronic acid, or salt thereof, crosslinked with divinylsulfone (DVS), said method comprising the steps of (a) providing an alkaline solution of hyaluronic acid, or salt thereof; (b) adding DVS to the solution of step (a), whereby the hyaluronic acid, or salt thereof, is crosslinked with the DVS to form a gel; (c) treating the gel of step (b) with a buffer, wherein the gel swells and forms a hydrogel comprising hyaluronic acid, or salt thereof, crosslinked with DVS.
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