METHOD FOR IMPROVED QUANTIFICATION OF MIRNAS
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United States of America Patent
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Jul 2, 2015
app pub date -
Jun 26, 2013
filing date -
Jun 27, 2012
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Abstract
The invention relates to a method and kit for enriching miRNA nucleic acids molecules of interest in a sampe by selective removal of nucleic acid species, in particular miRNA species, which are not of interest. The invention is based on the surprizing and previously unknown information that only a few individual miRNA species make up the majority of all miRNA species in a sample. Selective removal of these species prior to detection of miRNA nucleic acids molecules will improve assay time, assay sensitivity, and reproducibility.
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