Detection of unlabeled nucleic acids by changing solubility of surface-associating probes
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United States of America Patent
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May 7, 2019
Grant Date -
May 21, 2015
app pub date -
Nov 13, 2014
filing date -
Nov 13, 2013
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Abstract
Provided are methods and devices for detection of unlabeled nucleic acids. The detection methods are based on change of solubility of hydrophobic probes upon hybridization with a polynucleotide. In one embodiment, the probes are morpholino probes, having a fluorophore attached thereto. The morpholino probes are immobilized on a substrate that has fluorescence quenching functionality.
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