SEQUENCE CAPTURE METHOD USING SPECIALIZED CAPTURE PROBES (HEATSEQ)
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United States of America Patent
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Issued Date -
May 21, 2015
app pub date -
Jul 23, 2014
filing date -
Aug 2, 2013
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Abandoned
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Abstract
The present invention is a novel protocol for the massively parallel production of improved MIPs. The molecular improvements to the MIP cover the manufacturing of the probes, the workflow, the addition of unique sequence elements which connote sample specificity, and a sequence tag which uniquely identifies a specific molecule present in the initial sample population. Lastly, this invention also is combined with an empirical optimization strategy that overcomes issues of both locus representation and allelic bias. This improved technique is scalable and can be utilized to amplify targets comprised of a single locus' amplicon up to targeting more than 1 million loci.
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