Analysis of microbes by MALDI mass spectrometry
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United States of America Patent
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Oct 27, 2015
Grant Date -
May 21, 2015
app pub date -
Jun 6, 2013
filing date -
Jun 8, 2012
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Abstract
The invention relates to methods for the mass spectrometric analysis of microbes, in particular to a transfer method of microbes that are required to be identified from agar plates onto mass spectrometric sample supports and their preparation for ionization by matrix-assisted laser desorption (MALDI). Microbes from microcolonies which have grown on the agar plates after a culture time of only six to eight hours are transferred with a high transfer yield onto contact surfaces of suitable size by direct contact and cell disrupted on the contact surface; the released proteins are prepared with matrix material on the contact surface as MALDI samples. The ionization by matrix-assisted laser desorption also takes place on the contact surface.
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