BIOTINIDASE ASSAYS
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United States of America Patent
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Issued Date -
Apr 23, 2015
app pub date -
May 7, 2013
filing date -
May 7, 2012
priority date (Note) -
Abandoned
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Abstract
The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing.
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