Digital analysis of nucleic acid modification
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United States of America Patent
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Feb 13, 2024
Grant Date -
Apr 9, 2015
app pub date -
Oct 3, 2014
filing date -
Oct 4, 2013
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Abstract
In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.
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