METHODS FOR DETERMINING AND/OR MONITORING CONDITIONS OF A THREE DIMENSIONAL CELL CULTURE SYSTEM AND OPTICAL SENSOR DEVICE FOR CONDUCTING SAID METHODS
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United States of America Patent
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Issued Date -
Apr 2, 2015
app pub date -
Apr 26, 2013
filing date -
Apr 26, 2012
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Abandoned
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Abstract
A method for at least one of determining and monitoring at least one condition of/in a three dimensional cell culture system comprising at least one growth section, the at least one condition being selected from the group consisting of a physiological condition, a vitality, and a metabolism status, the method includes determining the physiological condition using erythrocytes as detectors, determining and/or monitoring the vitality by measuring living cell fluorescent dyes, and determining and/or monitoring the metabolism status by at least one of measuring an autofluorescence of NADH and/or FAD, by determining a NADH/NAD+ ratio, and by determining a NADH/FAD ratio.
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