ISFET ARRAY FOR DETECTING A SINGLE NUCLEOTIDE POLYMORPHISM
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United States of America Patent
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Apr 2, 2015
app pub date -
Mar 19, 2013
filing date -
Mar 30, 2012
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Abstract
Apparatus comprising a module for detecting a single nucleotide polymorphism, SNP, within a genome and comprising: four reaction chambers for receiving a fluid sample, the reaction chambers each containing a different one of the bases A, C, G, T, and a primer; a reference electrode which in use is immersed in said fluid sample; a pair of Ion Sensitive Field Effect Transistors, ISFETs, associated with each reaction chamber, the ISFETs of each pair comprising respective sensing membranes, or a common sensing membrane, exposed within the associated reaction chamber; and a plurality of difference detectors, each difference detector having a pair of inputs coupled respectively to outputs of ISFETs associated with different reaction chambers.
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