Nanopore Control With Pressure and Voltage
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United States of America Patent
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Sep 12, 2014
filing date -
Mar 13, 2012
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Abstract
There is provided a nanopore system including a nanopore in a solid state membrane. A first reservoir is in fluidic connection with the nanopore, the first reservoir being configured to provide, to the nanopore, nucleic acid molecules in an electrolytic solution. A second reservoir is in fluidic connection with the nanopore, with the nanopore membrane separating the first and second reservoirs. A pressure source is connected to the first reservoir to apply an external pressure to the first reservoir to cause nanopore translocation of nucleic acid molecules in the solution in the first reservoir. A voltage source is connected between the second and first reservoirs, across the nanopore, with a voltage bias polarity that applies an electric field counter to the externally applied pressure. Force of the externally applied pressure is greater than force of the electric field during nanopore translocation by the nucleic acid molecules.
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