DIRECTED DNA CO-POLYMER MASS AMPLIFICATION FOR ssDNA DETECTION
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Issued Date -
Feb 19, 2015
app pub date -
Jul 18, 2011
filing date -
Jul 19, 2010
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Abandoned
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Abstract
The disclosure generally relates to the use of a bio-barcode (BBC) method for capture and demonstrates hybridization co-polymerization amplification readout of the BBC as a method of rapid detection of single stranded DNA output with an increased sensitivity for the initial target input to the BBC assay. The BBC assay reporter ssDNA can be modified for rapid readout. Two ssDNA molecules are designed to co-polymerize and then continually hybridize into double stranded DNA. The double stranded DNA can be optically and/or electrically detected. Kits related to the two ssDNA molecules and the BBC assay also are disclosed.
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