Process for Ex Vivo Expansion of Stem Cells in a Bioreactor
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United States of America Patent
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Issued Date -
Feb 19, 2015
app pub date -
Mar 19, 2013
filing date -
Mar 23, 2012
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Abandoned
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Abstract
The present invention refers to a process of ex vivo expansion of stem cells, in a bioreactor, in particular hematopoietic stem/progenitor cells co-cultured with mesenchymal stem cells immobilized on microcarriers, for transplantation. The process comprises the steps of: a) forming a suspension of mesenchymal stem cells immobilized on microcarriers, b) inoculating in a bioreactor containing an expansion medium, hematopoietic cells co-cultured with mesenchymal stem cells immobilized on microcarriers c) expansion of hematopoietic cells. The process of the invention is capable of being implemented in a Kit.
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