METHOD FOR DETECTING DNA HAVING MICROSATELLITE REGION

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United States of America Patent

APP PUB NO 20150050649A1
SERIAL NO

14387097

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Abstract

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The invention provides a method of detecting DNA having a microsatellite region without causing the problem of a non-specific reaction product. The method includes (1) contacting a probe, which does not have a nucleotide sequence complementary to the microsatellite region and hybridizes with both sides of the nucleotide sequences of the microsatellite region, with DNA having the microsatellite region, to form a hybrid of the DNA and the probe, which has a loop structure including a microsatellite region, (2) separating the obtained hybrid, (3) detecting the hybrid. The invention also provides a hybrid of DNA and a probe, having a loop structure including a microsatellite region, which is made by contacting DNA having a microsatellite region with the probe which does not have a nucleotide sequence complementary to the microsatellite region, and hybridizes with both sides of the nucleotide sequence of the microsatellite region.

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Patent Owner(s)

Patent OwnerAddress
WAKO PURE CHEMICAL INDUSTRIES LTD1-2 DOSHOMACHI 3-CHOME CHUO-KU OSAKA-SHI OSAKA 540-8605
KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION2-22 HONCHO NAKA-KU KANAGAWA YOKOHAMA-SHI 2310005

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ishikawa, Tomokazu Amagasaki-shi, JP 260 2185
Kurosawa, Tatsuo Amagasaki-shi, JP 15 72
Matsukuma, Shoichi Yokohama-shi, JP 4 1

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