Importance

Loading Importance Indicators...

Abstract

An object of the present invention is to prepare individual microorganisms or a microflora composition derived from land or seafloor soil in which contamination of land or seafloor soil-derived substances is reduced. The present invention provides [1] a method for preparing, from a land or a seafloor solid samples, microorganisms containing a reduced level of land or seafloor soil-derived substances that inhibit nucleic acid analysis, which comprises (1) the step of disposing a suspension of a land or seafloor soil sample containing microorganisms and land or seafloor soil-derived substance that inhibit nucleic acid analysis as an electrolyte on a surface of a substrate at least a part of which constitutes an electrode, and applying a constant potential to the electrode to attach at least a part of the microorganisms to the surface of the substrate, and (2) the step of applying a high-frequency wave potential to the electrode to detach the microorganisms attached to the surface of the substrate in a viable state, and wherein the constant potential applied in the step (1) is greater than −0.5 V but not greater than −0.2 V (vs. Ag/AgCl), or greater than +0.2 V but not greater than +0.4 V (vs. Ag/AgCl), and the high-frequency wave potential applied in the step (2) has a frequency in the range of from 1 kHz to 20 MHz, and a potential range of ±0.1 V (vs. Ag/AgCl) or smaller.

Loading the Abstract Image...

First Claim