NON-EQUILIBRIUM TWO-SITE ASSAYS FOR LINEAR, ULTRASENSITIVE ANALYTE DETECTION
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United States of America Patent
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Issued Date -
Feb 12, 2015
app pub date -
Jan 11, 2013
filing date -
Jan 13, 2012
priority date (Note) -
Abandoned
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Abstract
Methods and kits related to non-equilibrium, ultrasensitive two-site assays for detecting analytes are provided. In one aspect, a two-site assays for detecting analytes under non-equilibrium analyte binding conditions, using low concentrations of reporter specificity molecule (e.g., reporter antibody) and kits for performing the same is provided. In another aspect, methods for selecting antibodies or specificity molecules with low dissociation constants for use as reporter antibodies in non-equilibrium two-site immunoassays, including two-site immuno-PCR assays, and assays performed with those antibodies, are also provided.
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