Methods and Compositions for Selective Labeling of Different Biotinylated Targets within Multicolor or Multilabel Assays
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United States of America Patent
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Jan 15, 2015
app pub date -
Jul 29, 2014
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Mar 2, 2011
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Disclosed are compositions and methods for the labeling of two or more targets with different labels. Specifically, disclosed are compositions for biotin and the protection of biotin within multilabel assays which employ the biotin-biotin binding protein binding relationship for each distinct label in relation to targets such as nucleic acids, polypeptides, antibodies or cells. These multilabel assays are enabled through the use of biotin with desthiobiotin, orthogonal protecting schemes for biotin, or a combination of the approaches.
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