METHOD FOR CULTURING ISLET CELLS AND METHOD FOR PREPARING CARRIER FOR ISLET CELL TRANSPLANTATION USING ATELOCOLLAGEN, AND ARTIFICIAL PANCREAS PREPARED USING SAME
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United States of America Patent
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Dec 25, 2014
app pub date -
Mar 4, 2013
filing date -
Mar 5, 2012
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The present invention provides a method of culturing pancreatic islet cells using a cationized atelocollagen prepared by ionization of high purity atelocollagen, a method of preparing a carrier for pancreatic islet cell transplantation using a cationized atelocollagen, and an artificial pancreas prepared using the same. According to the present invention, it is possible to increase the viability and/or glucose-dependent insulin secretion of pancreatic islet cells during culture of the cells by the use of a cationized atelocollagen or crosslinked atelocollagen scaffold obtained by ionization of high purity atelocollagen. In addition, it is possible to increase the viability and glucose-dependent insulin secretion of cultured and transplanted pancreatic islet cells by the use of a highly stable carrier for pancreatic islet cell transplantation that comprises cationized atelocollagen and alginate.
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