IN VITRO METHOD FOR MODIFYING THE DEPLETION PROFILE OF TREG CELLS PRESENT IN A TOTAL SPLENOCYTE POPULATION OF A BIOLOGICAL SAMPLE BY MEANS OF THE ISOLATION, CULTURING AND EXPOSURE THEREOF TO AN ATP AND POLYMIXIN B MEDIUM
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Feb 17, 2014
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Aug 18, 2011
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Abstract
In vitro method to modify the deplexion profile of Treg Cells present in a total splenocyte population of a biological sample comprising isolating, cultivating and submitting the splenocyte at a ATP medium and polymyx B, where the splenocytes at concentrations of Polymyxin B in the range 0.1 μg/ml to 100 μg/ml, and combined use of Polymyxin B and ATP to prepare a medicament useful in the treatment tumor or cancer mammals, where cancer is melamine.
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