Simultaneous Assay of Target and Target-Drug Binding
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United States of America Patent
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Issued Date -
Dec 4, 2014
app pub date -
Aug 6, 2014
filing date -
May 24, 2004
priority date (Note) -
Abandoned
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Abstract
Whole cell, simultaneous target and drug-target assay using differentially labeled antibodies and flow cytometry. First antibody binds to total target and second antibody binds to the drug binding site of the target, thus drug binding will competitively inhibit the second antibody allowing for a competitive inhibition assay of drug-target binding. The assay allows for whole cell analysis and even analysis of mixed populations of cells, yet provides detailed kinetic assessment of drug activity.
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