METHODS OF SCREENING MONOCLONAL ANTIBODY POPULATIONS
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United States of America Patent
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Dec 4, 2014
app pub date -
Jan 16, 2013
filing date -
Jan 16, 2012
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Abstract
The present invention concerns methods and compositions for screening primary hybridoma cultures to generate monoclonal antibodies that are useful in a variety of methods, including for in situ cellular imaging by immunocytochemical assays or in vivo applications, for example. Embodiments of the methods concern the use of automated high-throughput immunofluorescence (for example) to identify subcellular in vivo patterns that are expected based on the antigen or that may be unforeseen.
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