PRIMERS AND KITS FOR COLONY MULTIPLEX PCR FOR THE DETECTION OF CLASS A, B, C, AND D BETA-LACTAMASE GENES AND METHODS OF USING THEREOF
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United States of America Patent
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Nov 27, 2014
app pub date -
May 21, 2014
filing date -
May 21, 2013
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Abstract
The present invention provides kits and primers for colony multiplex PCR for the detection of class A, B, C, and D β-lactamase genes. The rapid detection of bla genes by using the kits and primers according to the present invention allows appropriate prescribing of antibiotics, which can reduce patient mortality and minimize antibiotic resistance. The present invention provides kits and primers for a rapid and accurate molecular method to overcome (a) to detect all clinically-important bla genes and (b) to explain phenotypic tests' results well by using 54 primer pairs, which are designed through novel and elaborate optimization processes. With perfect specificity and sensitivity in 172 control strains and 403 clinical strains, the present invention provides prompt and clinical application to the identification of all bla genes in bacterial pathogens.
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