Importance

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Abstract

A method for the detection of at least one analyte in a sample is disclosed, wherein it comprises the steps of: a) providing a layer comprising a porous matrix having at least one ligand bound thereto above a filter constituting the bottom of at least one well of an assay plate, wherein said filter does not allow passage of said porous matrix having a ligand bound thereto, b) adding a sample containing said at least one analyte to be detected, said at least one analyte having the ability to specifically bind to said at least one ligand, c) adding a wash solution with a view to washing out non-bound sample components from the assay plate through the filter, d) adding enzyme-linked antibodies or antigens having the ability to specifically bind to said at least one analyte, e) adding a wash solution with a view to washing out non-bound enzyme-linked antibodies or antigens from the analysis plate through the filter, f) adding a substrate specific for the enzyme, wherein a product is formed by a reaction between the enzyme and the substrate, determining the quantity of said at least one analyte by measuring a signal related to the product, as well as method for the diagnosis of an EHEC (enterohemorrhagic Escherichia coli) infection or HUS (hemolytic uremic syndrome), wherein a body sample from a patient is added as the sample and the analyte is detected using the above disclosed method, wherein the saccharide in the ligand is Galα1-Gal or Galα1-4Galβ1-4Glc.

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First Claim