COMPOSITIONS, KITS, AND RELATED METHODS FOR DETECTING AND/OR MONITORING SHIGA TOXIN PRODUCING ESCHERICHIA COLI
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United States of America Patent
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Nov 20, 2014
app pub date -
Dec 31, 2012
filing date -
Dec 30, 2011
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Abstract
ECF, such as the ecf operon/gene cluster (e.g., ECF2-1- and ECF2-2 described herein) may be used to detect virulent STECs including virulent non-0157:H7 STEC and virulent non-0157:H7 EHEC. Use of this nucleic acid target, in combination with other targets, such as Z5866, rfb0157, wzx0157, wzy0157, Z0344, Z0372, SIL0157, and katP junction provides a robust, sensitive assay for distinguishing 0157:H7 from virulent non-0157:H7 STEC. Compositions, kits and methods used for the detection of E. coli STEC are further disclosed. Certain E. coli sequences were surprisingly efficacious for the detection of 0157:H7 and virulent non-0157 STECs, such as the big six: 026, 045, 0103, 0111, 0121, and 0145.
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