Engineering an increase in ethanol production by altering cofactor specificity
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United States of America Patent
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Sep 8, 2020
Grant Date -
Oct 30, 2014
app pub date -
Nov 30, 2012
filing date -
Nov 30, 2011
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Abstract
The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
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