Apparatus for Improved Immunosuppressant Drug Monitoring
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United States of America Patent
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app pub date -
Jun 20, 2014
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Mar 14, 2013
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Abstract
A liquid chromatography/mass spectrometry system includes: a source of a first mobile phase solvent consisting essentially of water plus 10 mM ammonium formate plus 0.05% formic acid; a source of a second mobile phase solvent consisting essentially of methanol plus 10 mM ammonium formate plus 0.05% formic acid; a chromatography column comprising a length of 30 mm or less of a stationary phase comprising an 8-carbon alkyl chain material bonded to 2.6 μm diameter particles having solid silica cores surrounded by porous silica outer layers; an electrospray ion source of a mass spectrometer fluidically coupled to the chromatography column so as to generate ions therefrom; a mass analyzer of the mass spectrometer operable to quantitatively detect the ions; and a programmable processor electronically coupled to the mass analyzer and comprising instructions operable to determine, based on the ion detection, a concentration of everolimus, sirolimus, tacrolimus, or cyclosporin A.
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