METHOD, COMPOSITION AND KIT OF VISUALISATION AND CHARACTERIZATION OF THE NERVOUS SYSTEM BY COMBINING THE STAINING FOR METAL IMPREGNATION AND IMMUNOHISTOCHEMISTRY
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United States of America Patent
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Oct 2, 2014
app pub date -
Mar 14, 2012
filing date -
Mar 16, 2011
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Abstract
A method of visualization and characterization of the nervous system, which combines two techniques for tissue staining, mercuric impregnation and immunohistochemistry, is described. The method disclosed makes compatible one with the other procedures of staining by carrying them out on tissues subjected to adequate fixing procedures. Thus, the method makes feasible to combine morphometric analysis, which can typically be carried out with mercuric impregnation, with antigenic and/or neurochemical cellular characterization, which can typically be obtained with immunohistochemistry. The method has proven simple, reliable, and suitable for both conventional microscopy and confocal laser scanning microscopy.
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