TWIST-TIE OLIGONUCLEOTIDE PROBES
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United States of America Patent
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Issued Date -
Sep 11, 2014
app pub date -
Mar 8, 2013
filing date -
Mar 8, 2013
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Abstract
A composition comprising population of labeled oligonucleotides is provided herein. In some embodiments, the probes are of the formula: T1-V-T2, wherein: the nucleotide sequence of the V region varies in said population; the V regions of the different oligonucleotides hybridize to sites that are tiled across a sequence in a target nucleic acid; the T1 and T2 regions do not hybridize with said target nucleic acid; within each oligonucleotide of the population, the T1 and T2 regions are not complementary; and within each oligonucleotide of the population, the T1 region is complementary to the T2 region of at least one of other oligonucleotide of the population. Also provided is a method that comprises hybridizing the population of labeled oligonucleotides with a target nucleic acid to produce a complex.
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