METHOD FOR CULTURING PLURIPOTENCY-MAINTAINED SINGLY DISPERSED CELLS BY MEANS OF LAMINAR FLOW
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United States of America Patent
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Issued Date -
Sep 4, 2014
app pub date -
Oct 22, 2012
filing date -
Oct 21, 2011
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Abandoned
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Abstract
The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspect of the present invention aims to provide a cloning method for hESCs and hiPSCs based on culture of dispersed single cells utilizing shear stress. The above problem is solved by providing a method for culturing dispersed single pluripotent cells, wherein the dispersed single cells are cultured under laminar flow conditions.
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