QUANTITATIVE SOFT AGAR COLONY FORMATION ASSAY USING TETRAZOLIUM THAT GENERATES WATER-SOLUBLE FORMAZAN
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United States of America Patent
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Issued Date -
Sep 4, 2014
app pub date -
Oct 3, 2012
filing date -
Oct 3, 2011
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Abandoned
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Abstract
This invention provides a quantitative assay technique for soft agar colony formation that can be carried out rapidly with high accuracy. Specifically, this invention relates to a method for evaluation of cell survival comprising: a step of overlaying agar at the bottom of a vessel with agar containing cells, overlaying the agar containing cells with a medium and culturing the cells; a step of removing the medium, adding tetrazolium that produces water-soluble formazan and an electron carrier and culturing the cells; and a step of evaluating cell survival based on the color developed by the water-soluble formazan.
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