APPARATUS AND METHOD FOR LATERAL FLOW AFFINITY ASSAYS
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United States of America Patent
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Sep 4, 2014
app pub date -
Jul 19, 2012
filing date -
Jul 19, 2011
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Abstract
The invention provides apparatus for the quantitative analysis of an analyte in a sample, comprising (i) a solid phase; and (ii) a detector, wherein the surface of the solid phase comprises (a)a first position for sample application, and (b)a second position, distant from the first position, wherein a first molecule that binds to the analyte and is capable of releasing a detectable species is either deposited at the first position or is added to the sample prior to application to the LF membrane, and wherein a second molecule that binds to the analyte is immobilised at the second position, and wherein an enzyme is immobilised, co-located with the immobilised molecule at the second position, and wherein the detector is located in close proximity to the immobilised molecule at the second position.
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