METHODS FOR PREPARING SINGLE DOMAIN ANTIBODY MICROARRAYS
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United States of America Patent
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Issued Date -
Aug 21, 2014
app pub date -
Sep 20, 2011
filing date -
Sep 20, 2011
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Abstract
The present invention relates to a method for preparing sd Ab microarray comprising the step consisting of: —i) providing a host cell capable of expressing a biotinylation enzyme —ii) transforming said host cell with a nucleic acid encoding for a fusion protein wherein a single domain antibody is fused at its carboxy terminal end to a biotinylation peptide —iii) culturing said host cell in presence of biotin in such a way that said fusion protein and biotinylation enzyme are expressed, resulting in biotinylation of said fusion protein —iv) lysing said host cell as cultured at step iii) —v) spotting the lysate obtained at step iv) on a solid sup port coated with an agent selected from the group consisting of avidin, streptavidin and/or any art known derivative of these agents A further object of the invention relates to a sd Ab microarray obtainable by the method of the invention.
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