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Abstract

Reversed phase solid silica gel sorbent layers which can adhere with appropriate binders to a solid surface were developed. Pre-coated RP-rotors of different thickness were used These pre-coated RP-rotors can be used for Centrifugal Preparative Thin-layer Chromatography (CPTLC) using any appropriate device for Centrifugal Chromatography, like Chromatotron® or Cyclograph. Organic compounds including very polar ones can be separated and/or purified in a preparative scale from mixtures by these pre-coated RP-rotors, using different solvent systems. Also described is a new chromatographic separating device (hereinafter Chromato Rotor) and a method for separation of constituents from natural products or mixture of organic compounds using a centrifugal preparative chromatographic instrument with a separating element which contains a binder free sorbent layer packed or loaded between two supporting rotors and retained from moving outside by a porous filter. Only eluants pass through. ChromatoRotors with different sorbents with different dimensions and capacity can be prepared and used for centrifugal chromatographic separation. Virtually any classes of organic compounds including very polar natural products can be separated or purified from a mixture by using these rotors due to the unlimited combination of sorbents and mixture of mobile phases, including but not limited to aqueous-based solvents. These plates can also be used for CPTLC using any appropriate device for Centrifugal Chromatography like Chromatotron® or Cyclograph® instruments.

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First Claim