UBIQUITIN CHAIN ASSEMBLY
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United States of America Patent
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N/A
Issued Date -
Aug 7, 2014
app pub date -
Sep 24, 2012
filing date -
Sep 23, 2011
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
There is provided a method for producing free polyubiquitin chains linked through a single desired lysine residue, comprising the steps of: (a) selecting an E3 ubiquitin ligase enzyme which is homologous to mammalian HECT E3 ligases and possesses the desired lysine residue specificity; (b) incubating the E3 enzyme with an E1 ubiquitin activating enzyme, an E2 ubiquitin conjugating enzyme and monomeric ubiquitin; and (c) if undesired linkages are present, removing the undesired linkages by exposure to a DUB enzyme having the appropriate specificity.
First Claim
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