SIGNAL AMPLIFICATION FOR IMMUNOASSAYS BY USE OF AVIDIN-BIOTIN LINKAGES
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United States of America Patent
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Issued Date -
Aug 7, 2014
app pub date -
Jan 27, 2014
filing date -
Mar 4, 2011
priority date (Note) -
Abandoned
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Abstract
In sandwich-type immunoassays that capture a protein analyte between a capture antibody, typically bound to a solid phase, and a detection antibody that is coupled to a reporter group, the number of reporter groups associated with each molecule of analyte is increased by a variety of methods that utilize avidin-biotin-type binding in conjunction with such features as immunological binding to the reporter group on the detection antibody or multiple biotin-avidin-type binding sites.
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