Signal Ratio in Assay Calibrators
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United States of America Patent
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Issued Date -
Jul 31, 2014
app pub date -
Jan 30, 2013
filing date -
Jan 30, 2013
priority date (Note) -
Abandoned
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Abstract
Methods of enhancing signal ratio between calibrators in an assay for an analyte include conducting an assay for the analyte with zero concentration of analyte in a first calibrator to determine a first signal level. The reagents employed in the assay comprise an antibody reagent comprising an antibody for the analyte wherein a hinge region of the antibody is conjugated to a moiety. The assay for the analyte is also conducted with a second concentration of analyte in a second calibrator to determine a second signal level wherein the second analyte concentration is greater than zero and wherein the reagents employed in the assay comprise the antibody reagent. A ratio of the first signal level to the second signal level is determined and evaluated.
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