Glucanases, nucleic acids encoding them and methods for making and using them
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United States of America Patent
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Aug 23, 2016
Issued Date -
N/A
app pub date -
Sep 5, 2013
filing date -
Jul 2, 2003
priority date (Note) -
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Abstract
The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
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International Classification(s)
- C12P:FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESI....
- A23L:FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT....
- C12N:MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF
- A23K:FODDER
- G01N:INVESTIGATING OR ANALYSING MATERIALS BY DETERMININ....
- A23C:DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE; MILK OR....
- D21C:PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE ....
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