Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
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United States of America Patent
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Jul 17, 2018
Grant Date -
Jul 10, 2014
app pub date -
Dec 12, 2013
filing date -
Dec 12, 2012
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Abstract
The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, or portions, components, or subunits of bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.
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