PRIMER SET AND A METHOD FOR IDENTIFICATION OF MEAT SPECIES
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United States of America Patent
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Issued Date -
Jul 10, 2014
app pub date -
Dec 20, 2013
filing date -
Dec 22, 2012
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Abandoned
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Abstract
The invention discloses a primer set for identification of meat species. The primer set comprises a pair of outer primers used to amplify a sense strand of DNA fragment of mitochondrial cytochrome b between positions 51 and 507. The pair of outer primers comprises a forward outer primer and a backward outer primer. The primer set further comprises a pair of inner primers comprising a forward inner primer and a backward inner primer. The forward inner primer comprises a first annealing portion and a first warped portion. The backward inner primer comprises a second annealing portion and a second warped portion. Accordingly, the primer set is used to identify species of meat especially with DNA fragmentation due to processing processes of processed food.
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