METHODS AND COMPOSITIONS FOR MODULATION OF AMPLIFICATION EFFICIENCY
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United States of America Patent
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Jul 10, 2014
app pub date -
Mar 11, 2014
filing date -
Oct 31, 2012
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Provided herein are methods and kits for modulating the amplification efficiency of nucleic acids, which are useful in multiplex reactions where the amplification efficiency of one or more nucleic acids in the mixture are desired to be modulated relative to one or more other nucleic acids. Embodiments relate to molecular diagnostics, including detecting sequence variants, such as SNPs, insertions deletions, and altered methylation patterns, as well as the modulation of the amplification efficiency of internal control sequences to provide more accurate control sequences for amplification reactions.
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