LATERAL FLOW DETECTION OF TARGET SEQUENCES
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United States of America Patent
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Issued Date -
Jun 19, 2014
app pub date -
Sep 27, 2013
filing date -
Sep 27, 2012
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Abstract
Provided is a sensitive method to specifically detect nucleic acid sequences using a combination of DNA replication based signal amplification (e.g., PCR) and lateral flow analyte detection. The disclosed method uses a dual-labeled DNA probe complementary to a region of the target DNA sequence. Cleavage of the probe, caused by the presence of the amplified DNA target, is detected using lateral flow methods.
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