QUANTITATION OF HUMAN GENOMIC AND MITOCHONDRIAL DNA
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United States of America Patent
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Issued Date -
May 29, 2014
app pub date -
Sep 21, 2011
filing date -
Sep 21, 2010
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Abandoned
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Abstract
Methods are provided for determining, in a single polymerase chain reaction (PCR) reaction, the quantity, quality, and gender of origin of DNA in a sample, and whether or not the sample contains PCR amplification inhibitors. The methods involve carrying out a single PCR multiplex reaction utilizing primer sets specific for amplifying: the human amelogenin locus; an X- and/or Y-chromosome specific gene that is shorter than the amelogenin gene; at least one mitochondrial DNA sequence, and preferably two differently-sized mitochondrial sequences; and a heterologous, non-human reporter gene.
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