Expression of Hexose Kinase in Recombinant Host Cells
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United States of America Patent
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Issued Date -
May 22, 2014
app pub date -
Jan 24, 2014
filing date -
Dec 29, 2009
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Abandoned
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Abstract
The invention relates to a recombinant host cell having (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and optionally (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing glucose consumption, methods of improving redox balance, and/or methods of increasing the production of a product of a pyruvate-utilizing pathway.
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