METHODS FOR IMPROVING LIGATION STEPS TO MINIMIZE BIAS DURING PRODUCTION OF LIBRARIES FOR MASSIVELY PARALLEL SEQUENCING
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United States of America Patent
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May 8, 2014
app pub date -
Sep 27, 2013
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Sep 27, 2012
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Abstract
Described herein is a thermostable enzyme capable of efficient ligation of two oligomers at high temperature. The embodiments herein have led to the development of an optimized ligation step used in library preparation for sequencing reactions.
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