Cell separation method using a release system for cell-antibody-substrate conjugates containing a polyethylene glycol spacer unit
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United States of America Patent
Stats
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N/A
Issued Date -
Apr 24, 2014
app pub date -
Oct 2, 2013
filing date -
Oct 23, 2012
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
The invention is directed to a releasable conjugate comprising a biotinylated ligand having a biotin moiety, a ligand moiety (Ligand1) and a biotin-binding molecule (bbm) bound to the biotin moiety of the biotinylated ligand. The ligand moiety of the biotinylated ligand may be separated by a spacer group consisting of polyethylene glycol. Furthermore, the invention relates to a method for cleaving the releasable conjugate by providing biotin or streptavidin and an auxiliary release agent in a sufficient concentration to displace the biotin-binding molecule (bbm) from the biotin moiety of the biotinylated ligand and a method for separation of target cells from a cell sample utilizing the conjugate.
First Claim
Other Claims data not available