METHODS OF ISOLATING CELLS
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United States of America Patent
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Issued Date -
Apr 17, 2014
app pub date -
Apr 30, 2012
filing date -
Apr 29, 2011
priority date (Note) -
Abandoned
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Abstract
Provided herein are methods of isolating and expanding a plurality of multipotent cells (e.g., MSCs and ASCs) using a first culture in a suitable 2-dimensional substrate and a second culture in a suitable 3-dimensional substrate containing the basement membrane component and ROCK inhibitors. Also described are multipotent cell cultures made by the methods. Multipotent cell cultures as described may be used for transplantation or as niche cells for supporting other types of stem cells.
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