High pressure sperm sorting and flow cytometer methods
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United States of America Patent
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Apr 14, 2020
Grant Date -
Apr 10, 2014
app pub date -
Oct 3, 2013
filing date -
Oct 5, 2012
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Abstract
Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.
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