Expansion Medium for CD34-Negative Stem Cells
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United States of America Patent
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Issued Date -
Mar 27, 2014
app pub date -
Sep 11, 2013
filing date -
Dec 23, 2009
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Abstract
This invention provides a cell growth medium comprising (a) a human platelet lysate free of solid matter greater than 0.22 μm in diameter; (b) a human fresh frozen plasma (FFP) filtrate free of solid matter greater than 0.22 μm in diameter; (c) heparin; (d) L-glutamine; and (e) a serum-free, low glucose medium suitable for mammalian cell growth, wherein the cell growth medium permits the expansion of human CD34− stem cells and wherein the resulting expanded CD34− stem cells retain the ability to differentiate. This invention also provides related cell growth medium supplements, a sterile human platelet lysate and human fresh frozen plasma (FFP) filtrate, kits, CD34− stem cell-containing compositions, and related production and cell expansion methods.
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