LARGE, HS6ST2 OR ST8SIA1 ACTIVATORS FOR PREVENTING AND/OR ATTENUATING SKIN AGEING AND/OR HYDRATING SKIN
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United States of America Patent
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app pub date -
May 15, 2012
filing date -
May 17, 2011
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Abstract
The invention relates to the identification and the use of compounds which activate the expression of at least one gene selected from LARGE, HS6ST2 and ST8SIA1, for preventing and/or attenuating ageing, and/or for hydrating skin. The invention thus relates to an in vitro method for screening for candidate compounds for preventing and/or attenuating ageing of the skin, and/or for hydrating the skin, including the following steps: a. bringing at least one test compound in contact with a sample of keratinocytes; b. measuring the expression of at least one gene selected from LARGE, HS6ST2 and ST8SIA1, in the keratinocytes; c. selecting the compounds for which an activation of at least 1.4 fold of the expression of at least one of the genes is measured in the keratinocytes treated in a. compared with the untreated keratinocytes.
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