METHODS OF PURIFYING POLYPEPTIDES
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United States of America Patent
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Issued Date -
Mar 20, 2014
app pub date -
Dec 15, 2010
filing date -
Dec 18, 2009
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Abstract
The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an ion-exchange matrix relative to the amount of one or more impurities bound to the ion-exchange matrix. This effect is achieved by adding a chemical compound in the process which by also binding to the ion-exchange matrix due to a change that is opposite to the change of the ion-exchange matrix, reduces the binding of impurities more than the binding of the polypeptide of interest.
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